Zebrafish Tol2 transgenesis is a method that allows the introduction of specific genetic modification into zebrafish, a widely used model animal in biomedical research. Utilizing the Tol2 transposon system for transposon insertion, we generate transgenic zebrafish lines featuring the desired genetic alterations, offering unparalleled insights into human health and disease.
At InVivo Biosystems, our advanced Zebrafish Tol2 transgenesis services, including Tol2 plasmid preparation and the creation of humanized zebrafish models, will revolutionize the way scientists explore and understand complex biological processes.
More Zebrafish CRO Services
Compared to other methods, inserting large content in the zebrafish genome using the Tol2 transposon-based system offers advantages for integrating larger DNA fragments into the zebrafish genome. This system allows researchers to introduce and study genes, regulatory elements, or other genetic elements that may be relatively large in size, providing opportunities to investigate complex genetic interactions and gene functions.
Leveraging the Tol2 transposon system, derived from medaka fish, we provide a reliable and efficient method for creating transgenic zebrafish models that enable you to:
Comparison Of Tol2 Vs. CRISPR
Tol2 Transposase
Random insertion
Multiple
Yes
10% to 40%
Transgenesis
Mosaic
Usually 13-15kb - depends on cargo
CRISPR/ Cas9
Site specific
Single
Yes
~2% to 5%
Knock-in Edit
Mosaic
Unknown but appears that the larger the insert the lower the efficiency.
Tol2 Plasmid Prep
Tol2 Plasmid Validation
Mosaic Clutch* (F0 injected embryos)
Full Build** (Stable transgenic line)
Tol2 Plasmid Design
Plasmid Assembly
InVivo Integration Assessment
Expertly Injected Embryos
Germline Transmission Screening & Line Propagation
* This service is only available to clients in the United States.
** Full Build includes screening n=100 adults; standard husbandry charge applies.
# Additional charge applies for cargo over 2kb in size for all service packages.
The zebrafish Tol2 transgenesis service is a specialized service that enables the efficient and precise integration of transgenes into the zebrafish genome using the Tol2 transposon system.
The zebrafish Tol2 transgenesis service is a specialized service that enables the efficient and precise integration of transgenes into the zebrafish genome using the Tol2 transposon system.
The zebrafish Tol2 transgenesis service is a specialized service that enables the efficient and precise integration of transgenes into the zebrafish genome using the Tol2 transposon system.
The Tol2 transposon system utilizes a DNA sequence called the Tol2 transposon, which can "jump" or transpose from one location to another in the zebrafish genome edits. This system allows for the stable and heritable integration of transgenes.
The service offers several benefits, including high efficiency in generating transgenic zebrafish, precise insertion of transgenes or expression cassettes at desired genomic locations, and the ability to study gene function, disease models, and developmental processes.
The timeline for the service may vary depending on the specific requirements and complexity of the project. Generally, the process involves plasmid construction, microinjection into zebrafish embryos, and subsequent screening and validation. The duration can range from a few weeks to a couple of months.
Yes! The service allows for the integration of custom transgenes, including reporter genes, fluorescent markers, or genes of interest. Researchers can provide their own transgene constructs, and the service will facilitate their integration into the zebrafish genes.
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